Sunday Evening News 428 - Weeks 23/24 - 2025
Weekly report on genetic engineering, genome editing, biotechnology and legal regulation.
June 2025-06-02 - June 2025-06-15
Meetings – Conferences / Treffen - Veranstaltungen
Erinnerung:
Die Arbeitsgruppe Gentechnologiebericht am BIH at Charité richtet am 24. Juni 2025 eine öffentliche Abendveranstaltung zur Pflanzengenomeditierung aus.
Die Genschere in München, 25.6. bis 28.6.2025
https://www.crispr-whisper.de/2025/04/23/die-genschere-in-muenchen-25-6-bis-28-6-2025/
______________________________________________
„Ohne Gentechnik“-Konferenz VLOG: Forum am 03.07.2025 in Berlin
https://www.ohnegentechnik.org/artikel/vlog-forum2025
Anmeldung zum ÖkoProg-Event - 26. Juli 2025 ab 12 Uhr in Alpen am Niederrhein
https://docs.google.com/forms/d/e/1FAIpQLSdaJmcT8vew5dDr806IubUw4duxuv-3GqkwH3XwKkcJmnU1ag/viewform
Non-GM Soy Conference 2025 in Frankfurt; 04. Nov. 2025:
Ab sofort ist die Anmeldung für die Non-GM Soy Conference 2025 möglich – alle Infos dazu gibt es auf www.nongmomarket.com
https://gentechnikfrei.at/4-nov-2025-non-gm-soy-conference-2025-in-frankfurt/
Press Releases - Media / Presse- und Medienberichte
Grimm V.: „Die Fixierung der EU auf Risikovermeidung wird immer mehr zum Wettbewerbsnachteil“
Neue Gentechnik: Alles nur Hype?
https://gentechfrei.ch/de/themen/neue-gv-verfahren/5478-neue-gentechnik-alles-nur-hype
Bericht/Report: New GMOs Market Report | Edition 01 | June 2025
https://gentechnikfrei.at/wp-content/uploads/2025/06/New-GMOs-Market-report-2025_05-06-25.pdf
Non-GMO Project Staff: New GMO Alert: GMO Rice Isn't on the Market, but It's Still Showing Up
Glyphosat: EU-Kommission will neue Studie zu Krebsrisiko prüfen lassen
Bergoënd A.: Commission to seek review of new data linking glyphosate to higher cancer risk
Hussendörfer E.: Die revolutionäre Technologie, auf die Deutschland freiwillig verzichtet
ETC Group & GRAIN: Top 10 agribusiness giants: corporate concentration in food & farming in 2025
China approves new food materials and additives including three GM microorganisms
Only some selected press releases or media reports are listed here. The daily up-date of the press releases and
media reports are ►here: June, week 23, 24
Publications – Publikationen
Kutubuddin A. Molla K.A., Moronta-Barrios F., Karembu M., De Haro,L.P., Jannah R (2025): The Spirit of Asilomar: lessons for the
next era of biotechnology governance. Trends in Biotechnology. TIBTEC 2712
The first Asilomar Conference on Recombinant DNA, held in February 1975, marked a unique moment in scientific history, when researchers took collective responsibility to set guidelines for their groundbreaking work. Fifty years later, as technologies like CRISPR, synthetic biology, mirror life, and artificial intelligence (AI)-driven bioengineering open unprecedented possibilities, a diverse group of stakeholders gathered again at the historic Asilomar Conference Grounds in Pacific Grove, California. This 50th anniversary summit, The Spirit of Asilomar and the Future of Biotechnology, brought together scientists, journalists, regulators, ethicists, policymakers, public representatives, voices from the Global South, and the Next Generation Leaders to reflect on governance at a time when humanity holds the tools to reshape itself. From February 23 to 26, 2025, nearly 300 participants, including a few attendees of the original 1975 meeting, discussed how best to govern rapidly evolving and powerful biotechnologies. Today’s landscape is far more complex – with genome editing, synthetic biology, gene drives, AI integration, corporate interests, and cross-border implications – posing new challenges for governance. Models developed 50 years ago may no longer suffice. In this TrendsTalk, a few summit participants offer a timely and thoughtful perspective on the future of biotechnology governance.
https://www.cell.com/trends/biotechnology/abstract/S0167-7799(25)00179-9
Defraeye T., Bahrami F., Kowatsch T., Annaheim S. et al (2025): Advances in Food-As-Medicine Interventions and Their Impact
on Future Food Production, Processing, and Supply Chains. Advances in Nutrition 16 (6), 100421 | https://doi.org/10.1016/j.advnut.2025.100421
Food-as-medicine (FAM) is an emerging trend among medical doctors, health insurers, startups, and governmental public-health and nongovernmental organizations. FAM implies using food as a part of an individual’s health plan to prevent or help treat acute and chronic health conditions and diseases. We highlight trends and hurdles in the FAM intervention pyramid. Our viewpoint is to indicate how interventions might change the future demand for specific food groups, their transport in supply chains, and the technologies used to process them. On the basis of national guidelines, dietary interventions can help to prevent and treat many diseases, including cardiovascular disease, cancers, type 2 diabetes, and obesity. FAM R&D and services offer more individualized treatments. This is challenging given the interindividual variability and complexity of the body’s response to food and related factors, such as dietary habits, genetics, lifestyle, and biosphere. Quantifying health improvements is essential to prove the added value of more individualized FAM interventions compared with adopting a general healthy diet. It is unclear which level of individualization of interventions produces the largest health benefits at the lowest costs for the patient, healthcare system, and climate. FAM interventions can support and complement conventional medical treatment. They will require a shift to producing more health-promoting foods, including whole foods, minimally processed foods, and selected processed foods. The food processing industry and supply chains must adapt to these new scenarios. Auxiliary technologies and methods are enablers, including delivery services, wearable technology, health-monitoring apps, and data-driven consumer behavior analysis.
https://www.sciencedirect.com/science/article/pii/S2161831325000572?via%3Dihub
Kossmann, H., Engeler, H.L., Breunig, P. et al. (2025): New Genetic Technologies (NGTs) as Mirrored by Mass Media - Topics
and Frames of Genetic Editing in German Press. Food ethics 10, 16 | https://doi.org/10.1007/s41055-025-00175-z
This study examines the portrayal of New Genetic Technologies (NGTs), particularly CRISPR/Cas9, in German mass media from 2012 to 2023 — a period marked by significant advancements in biotechnology and pivotal regulatory developments within the European Union. Recognizing the media’s critical role in shaping public perception and affecting policy, we analyzed articles from four major German newspapers: Frankfurter Allgemeine Zeitung, Süddeutsche Zeitung, taz, and DIE WELT. Employing topic modelling and frame analysis, we identified prevailing topics and interpretative frames within the coverage, thereby assessing broader trends in issue coverage and particular ways media portrayed NGT adoption in the agricultural sector as well as the food industry. In the course of our analysis, we first find notable media interest and an increasing share of articles reporting on the latter. Second, the topic model uncovered two dominant topics, which we labelled accordingly: agriculture & food production and medicine & human health, with the former likewise gaining prominence in recent years. Third, our frame analysis indicated that positive frames — particularly progress and agricultural productivity — were more prevalent than critical frames like ecology and public rejection. Following a historically critical media perspective surveyed by the existing literature on traditional genetic technologies, our findings suggest a media shift towards a more favorable portrayal of NGTs, especially in the context of agriculture. In light of these results, our study underscores the media’s potential influence on public attitudes and regulatory outcomes concerning NGTs.
https://link.springer.com/article/10.1007/s41055-025-00175-z
Panzacchi, S., Tibaldi, E., De Angelis, L. et al. (2025): Carcinogenic effects of long-term exposure from prenatal life to
glyphosate and glyphosate-based herbicides in Sprague–Dawley rats. Environ Health 24, 36 | https://doi.org/10.1186/s12940-025-01187-2
Background: Glyphosate-based herbicides (GBHs) are the world’s most widely used weed control agents. Public health concerns have increased since the International Agency for Research on Cancer (IARC) classified glyphosate as a probable human carcinogen in 2015. To further investigate the health effects of glyphosate and GBHs, the Ramazzini Institute launched the Global Glyphosate Study (GGS), which is designed to test a wide range of toxicological outcomes. Reported here are the results of the carcinogenicity arm of the GGS.
Methods:Glyphosate and two GBHs, Roundup Bioflow used in the European Union (EU) and RangerPro used in the U.S., were administered to male and female Sprague–Dawley (SD) rats, beginning at gestational day 6 (via maternal exposure) through 104 weeks of age. Glyphosate was administered through drinking water at three doses: the EU acceptable daily intake (ADI) of 0.5 mg/kg body weight/day, 5 mg/kg body weight/day and the EU no-observed adverse effect level (NOAEL) of 50 mg/kg body weight/day. The two GBH formulations were administered at the same glyphosate-equivalent doses.
Results:In all 3 treatment groups, statistically significant dose-related increased trends or increased incidences of benign and malignant tumors at multiple anatomic sites were observed compared to historical and concurrent controls. These tumors arose in haemolymphoreticular tissues (leukemia), skin, liver, thyroid, nervous system, ovary, mammary gland, adrenal glands, kidney, urinary bladder, bone, endocrine pancreas, uterus and spleen (hemangiosarcoma). Increased incidences occurred in both sexes. Most of these involved tumors that are rare in SD rats (background incidence < 1%) with 40% of leukemias deaths in the treated groups occurring before 52 weeks of age and increased early deaths were also observed for other solid tumors.
Conclusions: Glyphosate and GBHs at exposure levels corresponding to the EU ADI and the EU NOAEL caused dose-related increases in incidence of multiple benign and malignant tumors in SD rats of both sexes. Early-life onset and mortality were observed for multiple tumors. These results provide robust evidence supporting IARC’s conclusion that there is “sufficient evidence of carcinogenicity [of glyphosate] in experimental animals”. Furthermore, our data are consistent with epidemiological evidence on the carcinogenicity of glyphosate and GBHs.
https://ehjournal.biomedcentral.com/articles/10.1186/s12940-025-01187-2
Dublino R., Ercolano M. (2025): Artificial intelligence redefines agricultural genetics by unlocking the enigma of genomic
complexity. The Crop Journal | https://doi.org/10.1016/j.cj.2025.05.008
Artificial intelligence (AI) has transformed agricultural genetics, especially in the context of crop improvement strategies. Traditional breeding faces challenges such as polyploidy, high level of genomic heterogeneity, and complex gene-trait associations. By combining multi-omics data researchers learn more about the genetic and molecular basis of important agricultural traits. However, statistical methods are often insufficient to address the data complexity. By contrast, AI techniques, such as machine learning (ML) and deep learning (DL), are emerging as powerful tools to explore complexity. Algorithms such as random forests (RF) and support vector machines (SVM) can support genomic selection (GS) and trait value prediction. Furthermore, DL models such as convolutional neural networks (CNN) and long short-term memory networks (LSTM) dominate high-throughput phenotyping and time series analyses, providing accurate predictions for crop yield, disease resistance, and genotype adaptation. Large language models (LLMs) are able to integrate complex omics data. AI models can analyze large dataset, generated by genomics, transcriptomics, proteomics, metabolomics, and phenomic applications because algorithms can combine different inputs, such as DNA sequences, gene expression profiles, protein–protein interaction networks, metabolite concentrations, and phenotypic data under specific environmental conditions. The integration of individual models can improve prediction accuracy by reducing resource inputs and automating labor-intensive tasks involved in breeding programs. Some recent AI methods, such as gradient boosting machines (GBMs) and Transformer models, are increasingly being used to improve scalability and accuracy of predictive analytics. This review summarizes major advances in AI applications in agricultural genetics, highlighting the strengths and limitations of different ML and DL models and their role in integrating complex datasets. The study highlights the importance of artificial intelligence in understanding genomic complexity and promoting the development of innovative methods to improve crop performance.
https://www.sciencedirect.com/science/article/pii/S2214514125001370
Basavantraya N. Devanna B.N., Arra Y., Madhav M.S. (2025): Editorial: Plant breeding innovations—CRISPR as a powerful
weapon for agricultural crops. Front. Genome Ed., Sec. Genome Editing in Plants Volume 7 - 2025 | https://doi.org/10.3389/fgeed.2025.1623540
https://www.frontiersin.org/journals/genome-editing/articles/10.3389/fgeed.2025.1623540/abstract
Tuncel, A., Pan, C., Clem, J.S. et al. (2025): CRISPR–Cas applications in agriculture and plant research.
Nat Rev Mol Cell Biol 26, 419–441 | https://doi.org/10.1038/s41580-025-00834-3
Growing world population and deteriorating climate conditions necessitate the development of new crops with high yields and resilience. CRISPR–Cas-mediated genome engineering presents unparalleled opportunities to engineer crop varieties cheaper, easier and faster than ever. In this Review, we discuss how the CRISPR–Cas toolbox has rapidly expanded from Cas9 and Cas12 to include different Cas orthologues and engineered variants. We present various CRISPR–Cas-based methods, including base editing and prime editing, which are used for precise genome, epigenome and transcriptome engineering, and methods used to deliver the genome editors into plants, such as bacterial-mediated and viral-mediated transformation. We then discuss how promoter editing and chromosome engineering are used in crop breeding for trait engineering and fixation, and important applications of CRISPR–Cas in crop improvement, such as de novo domestication and enhancing tolerance to abiotic stresses. We conclude with discussing future prospects of plant genome engineering.
https://www.nature.com/articles/s41580-025-00834-3
Mudge S.; Olayemi M. (2025): Use of genetic manipulation and gene editing in the Australian sugarcane industry:
advances, relevance and barriers. Proceedings of the Australian Society of Sugar Cane Technologists, volume 46, 5-15
Several significant challenges threaten the future prosperity of the Australian sugarcane industry, and technologies such as genetic modification (GM) and, potentially, gene editing (GE) offer solutions to some of these problems. Adoption of these technologies, however, raises complex ethical, environmental and socioeconomic issues that would need to be carefully considered and addressed. We provide a summary of recent advances in GM and GE technologies (both in a broad sense and specifically in sugarcane), identify areas where strategic investment in GM and GE might be most relevant, and provide an assessment of current barriers to adoption for these technologies. The intent is to stimulate discussion andprovide a foundation for informed decision-making and strategic planning in the industry.
https://sugarresearch.com.au/wp-content/uploads/2025/05/Mudge-Olayemi.pdf
Mohammed, F.Y., Khonje, M.G. & Qaim, M. (2025): Women’s roles in decision-making and nutrition-sensitive agriculture.
Food Sec. | https://doi.org/10.1007/s12571-025-01553-5
The small-farm sector is home to many of the world’s food-insecure and undernourished people. Strategies to make smallholder farming more nutrition-sensitive often focus on agricultural diversification. In addition, women’s empowerment is widely considered useful to improve diets and nutrition. Many studies have analyzed the effects of farm production diversification and of women’s empowerment on dietary outcomes, but mostly in separate strands of literature. Here, we connect these strands to contribute to a better understanding of the multifaceted links between farm production diversity, women’s roles in decision-making, and household diets. Using primary data from Malawi, we show that women’s decision-making is positively associated with farm production diversity and with household dietary diversity. Furthermore, women’s decision-making increases the positive association between farm production diversity and dietary diversity. We also differentiate between different domains of decision-making, including agricultural production, market sales, cash income control, and food purchases. The results suggest that strengthening women’s agency can make smallholder farming more nutrition-sensitive through multiple channels.
https://link.springer.com/article/10.1007/s12571-025-01553-5
Venkataraman S, Zaruk D and Hefferon K (2025): Recent developments, challenges and opportunities in genome editing for
crop science from a societal perspective. Front. Genome Ed. 7:1568072. | https://doi.org/10.3389/fgeed.2025.1568072
Genome editing has presented enormous potential in the fields of medicine and agriculture. Here, we explore the social and regulatory aspects of genome editing from the perspective of food security. We provide recent examples of crop genome editing successes. We discuss the current regulatory framework for genome edited crops in North America and Europe, and present how public perception can influence international policies and trade.
https://www.frontiersin.org/journals/genome-editing/articles/10.3389/fgeed.2025.1568072/full
Riesenberg, S., Kanis, P., Karlic, R. et al. (2025): Robust prediction of synthetic gRNA activity and cryptic DNA repair by
disentangling cellular CRISPR cleavage outcomes. Nat Commun 16, 4717 (2025). https://doi.org/10.1038/s41467-025-59947-0
The ability to robustly predict guide RNA (gRNA) activity is a long-standing goal for CRISPR applications, as it would reduce the need to pre-screen gRNAs. Quantification of formation of short insertions and deletions (indels) after DNA cleavage by transcribed gRNAs has been typically used to measure and predict gRNA activity. We evaluate the effect of chemically synthesized Cas9 gRNAs on different cellular DNA cleavage outcomes and find that the activity of different gRNAs is largely similar and often underestimated when only indels are scored. We provide a simple linear model that reliably predicts synthetic gRNA activity across cell lines, robustly identifies inefficient gRNAs across different published datasets, and is easily accessible via online genome browser tracks. In addition, we develop a homology-directed repair efficiency prediction tool and show that unintended large-scale repair events are common for Cas9 but not for Cas12a, which may be relevant for safety in gene therapy applications.
https://www.nature.com/articles/s41467-025-59947-0
Köster, T., Venhuizen, P., Lewinski, M., Petrillo, E. et al. (2025), At-RS31 orchestrates hierarchical cross-regulation of splicing
factors and integrates alternative splicing with TOR-ABA pathways. New Phytol. https://doi.org/10.1111/nph.70221
Alternative splicing is essential for plants, enabling a single gene to produce multiple transcript variants to boost functional diversity and fine-tune responses to environmental and developmental cues. Arabidopsis thaliana At-RS31, a plant-specific splicing factor in the Serine/Arginine-rich (SR) protein family, responds to light and the Target of Rapamycin (TOR) signalling pathway, yet its downstream targets and regulatory impact remain unknown.
To identify At-RS31 targets, we applied individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP) and RNAcompete assays. Transcriptomic analyses of At-RS31 mutant and overexpressing plants further revealed its effects on alternative splicing.
iCLIP identified 4034 At-RS31 binding sites across 1421 genes, enriched in CU-rich and CAGA RNA motifs. Comparative iCLIP and RNAcompete data indicate that the arginine/serine (RS) domain of At-RS31 may influence its binding specificity in planta, underscoring the value of combining in vivo and in vitro approaches. Transcriptomic analysis showed that At-RS31 modulates diverse splicing events, particularly intron retention and exitron splicing, and influences other splicing modulators, acting as a hierarchical regulator.
By regulating stress response genes and genes in both TOR and abscisic acid signalling pathways, At-RS31 may help integrate these signals, balancing plant growth with environmental adaptability through alternative splicing.
https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.70221
Chien C.-C., Seiko T., Muto C., Ariga H. Et al. (2025): A single domestication origin of adzuki bean in Japan and the evolution
of domestication genes. Science 388, Issue 6750 | DOI: 10.1126/science.ads2871
INTRODUCTION AND RATIONALE: The adzuki bean (Vigna angularis), with its characteristic red seed coat color, is a crucial legume of East Asian culinary culture. Although archaeological records suggest the earliest traces of cultivation in Japan, previous genetic evidence identified the highest genetic variation in central Chinese cultigens. Because the hunter-gatherer-fisher Jomon populations inhabited the Japanese archipelago before the arrival of the rice-growing Yayoi populations about three thousand years ago (3 kya), the spatiotemporal origin of adzuki domestication remains debated. Furthermore, the evolution of some domestication traits (e.g., pod shattering and seed coat color) cannot be traced by archaeological records, requiring input from genetics studies.
RESULTS: We investigated about 700 adzuki accessions, covering wild and cultigen populations across the species range. The wild populations of this species likely originated near the Himalayas and naturally spread to central China and Japan. We identified genetic evidence of domestication 3 to 5 kya in Japan, with subsequent cultigen expansion back to China, followed by hybridization with Chinese wild populations and generation of admixed cultigen population in southern China. This complex demography likely thwarted previous attempts to trace adzuki domestication origin, and our results explain previous archaeological and genetic observations.
Wild adzuki seed coats have mottled black pigment spots on a pale background, and most cultigens have a characteristic uniform red background without black spots. We identified one major effect gene for each of the two traits. The gain of red background was caused by an amino acid mutation in the anthocyanidin reductase gene VaANR1. Our molecular characterization of the wild and cultigen alleles confirmed that this amino acid mutation altered enzyme function, disrupting the processing of anthocyanidins and the accumulation of downstream colorless proanthocyanidins. For the locus conferring mottled black pigment spots, the wild-progenitor haplotype contained three homologous MYB transcription factor genes, and only the central copy, VaPAP1, expressed during seed development. In cultigens, nonallelic homologous recombination likely happened between the two flanking copies, deleting VaPAP1 and resulting in a chimeric copy not expressed during seed development.
We modeled their evolutionary trajectories with another gene, VaMYB26, whose frameshift mutation was previously shown to cause pod nonshattering in cultigens. Because all three traits have a Mendelian genetic basis, the time-course mutant allele frequency reflects the domestication phenotype evolution in this predominately self-fertilizing species. Mutant frequency of the three genes increased much earlier than 5 kya, suggesting that weak selection existed before the inferred wild-cultigen divergence time.
CONCLUSION: In this study, we resolved the complex demography of adzuki domestication, identified and validated key genes for domestication traits, and inferred the evolutionary trajectory of these traits, which could not be directly observed from archaeological records. These results complement the growing archaeological evidence that Jomons were not exclusively hunter-gatherer-fishers from the genetics perspective and support the recent archaeobotanical view that weak selection may have existed in local populations thousands of years before clear traces of sophisticated agricultural activities.
https://www.science.org/doi/10.1126/science.ads2871
Fajardo C., Macedo, M.; Buha, T.; De Donato, M.; Costas, B.;Mancera, J.M. (2025): Genetically Modified Animal-Derived
Products: From Regulations to Applications. Animals 15, 1570 | https://doi.org/10.3390/ani15111570
Biotechnological advances applied to the generation of genetically modified (GM) animals have shown the potential to develop innovative solutions for different challenges in key areas such as agriculture and human medicine. Despite its enormous potential, the deployment of genetic modification in animals, and its subsequent commercialization, does not meet the same public acceptance as GM plant-derived products, which are currently widely adopted around the world. In this review, we highlight the main examples of GM and gene-edited animal-derived products already approved by the FDA and discuss the regulatory context inherent to such processes, including the risk-based assessment analysis based on a case-by-case evaluation. Moreover, cases of GM animals already approved by other jurisdictions around the world are also discussed.
https://www.mdpi.com/2076-2615/15/11/1570
Kallergi, A., Asveld, L. (2024): “Business as usual”? Safe-by-Design Vis-à-Vis Proclaimed Safety Cultures in Technology
Development for the Bioeconomy. Sci Eng Ethics 30, 56 | https://doi.org/10.1007/s11948-024-00520-1
Safe-by-Design (SbD) is a new concept that urges the developers of novel technologies to integrate safety early on in their design process. A SbD approach could—in theory—support the development of safer products and assist a responsible transition to the bioeconomy, via the deployment of safer bio-based and biotechnological alternatives. Despite its prominence in policy discourse, SbD is yet to gain traction in research and innovation practice. In this paper, we examine a frequently stated objection to the initiative of SbD, namely the position that SbD is already common practice in research and industry. We draw upon observations from two case studies: one, a study on the applicability of SbD in the context of bio-based circular materials and, two, a study on stakeholder perceptions of SbD in biotechnology. Interviewed practitioners in both case studies make claims to a strong safety culture in their respective fields and have difficulties differentiating a SbD approach from existing safety practices. Two variations of this argument are discussed: early attentiveness to safety as a strictly formalised practice and early attentiveness as implicit practice. We analyse these perceptions using the theoretical lens of safety culture and contrast them to the aims of SbD. Our analysis indicates that professional identity and professional pride may explain some of the resistance to the initiative of SbD. Nevertheless, SbD could still be advantageous by a) emphasising multidisciplinary approaches to safety and b) offering a (reflective) frame via which implicit attentiveness to safety becomes explicit.
https://link.springer.com/article/10.1007/s11948-024-00520-1
Brand N., Stadler F.M., Hahn L., Borisova Z., Wefers D. (2025): Fermentative in situ synthesis of isomalto/malto-
polysaccharides in sourdough. Food Chemistry 488,144846, | https://doi.org/10.1016/j.foodchem.2025.144846.
Some sourdough-derived lactic acid bacteria were shown to fermentatively synthesize potentially prebiotic
isomalto/malto-polysaccharides (IMMPs) from maltodextrins. Therefore, the application of suitable starter cultures and adjusted fermentation conditions could be used to achieve a fermentative synthesis of IMMPs in sourdough, however, this has not been investigated yet. To monitor in situ IMMP synthesis, a new method based on endo-dextranase hydrolysis and quantification of released isomaltose was developed. Subsequently, it was applied to determine the amount of dextran equivalents (fermentatively formed α-1,6-linkages) in sourdoughs produced under different conditions. Of all seven investigated strains, Limosilactobacillus reuteri TMW 1.106 in combination with the addition of pregelatinized starch and starch-debranching enzymes produced by far the highest amount of IMMPs within 20 h of fermentation. Other changes in fermentation conditions did not significantly increase IMMP formation. However, applying adjusted conditions during sourdough fermentation may be used to increase the amount of potentially prebiotic carbohydrates in bread.
https://www.sciencedirect.com/science/article/pii/S0308814625020977?via%3Dihub
EFSA
FEZ Panel (2025): Safety evaluation of the food enzyme triacylglycerol lipase from the genetically modified Komagatella phaffii strain
LALL-LI2. EFSA Journal, 23(6), e9486. https://doi.org/10.2903/j.efsa.2025.9486
https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/j.efsa.2025.9486
FEZ Panel (2025): Safety evaluation of the food enzyme endo-1,4-β-xylanase from the genetically modified Trichoderma reesei strain
DP-Nzd72. EFSA Journal, 23(6), e9485. https://doi.org/10.2903/j.efsa.2025.9485
https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/j.efsa.2025.9485
FEZ Panel (2025). Safety evaluation of the food enzyme glucan 1,4-α-maltohydrolase from the genetically modified Escherichia coli
strain MLAVSC. EFSA Journal, 23(6), e9483. https://doi.org/10.2903/j.efsa.2025.9483
https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/j.efsa.2025.9483
FEZ Panel (2025): Safety evaluation of the food enzyme mannan endo-1,4-β-mannosidase from the non-genetically modified
Aspergillus niger strain AE-HCM. EFSA Journal, 23(6), e9488. https://doi.org/10.2903/j.efsa.2025.9488
https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/j.efsa.2025.9488